All in One. All the flexibility you need in a 5x Master Mix for multiplexing applications. NOW LYO READY!
+ Sensitive. More free volume. 5x concentration allows more volume for target specific primers and probes (multiplexing).
+ Robust. Uniform amplification. Up to 30 target multiplexing in real-time (customer feedback).
+ Fast TTR. No extraction needed. Reliable results with crude samples without extraction step, like blood.
+ Specific. No false amplification. Engineered Taq DNA polymerase more stable at room temperature. Aptamer-based hot-start prevents false amplification and provides a fast-start function.
+ Lyo ready. Contains all necessary excipients needed for freeze-drying. Can be used for lyophilization. Enables RT storage and shipping once dried. Can be freeze-dried by us. Learn More
For research use and further manufacturing.
In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.
- Probe based Real time qPCRs
- Real time multiplex detection qPCRs
- Direct real-time qPCRs (without sample extraction)
molecular species identification by DNA barcoding
multiplexed amplifications for NGS-based barcoding
The activity of PlexTaq® DNA polymerase is monitored and adjusted to a specific DNA polymerase activity using an artificial DNA template and a DNA primer. PlexTaq® 5x qPCR Multiplex Master Mix is tested in a real-time multiplex qPCR. Hereby, the product demonstrates linearity of amplification over a specified serial dilution of target DNA. Enzyme concentration is determined by protein-specific staining. Please inquire more information at email@example.com for the lot-specific concentration. No contamination has been detected in standard test reactions.
PlexTaq® 5x qPCR Multiplex Master Mix is produced to our highest internal quality guidelines and within the scope of the actively held ISO 13485 quality management system certification.
Detection of three specific pathogen targets and an internal control. Total input DNA per reaction was 10^6 (red), 10^5 (yellow), 10^4 (blue), 10^3 (green), 10^2 (purple), 10 (light blue) and 0 copies (grey) and 8000 copies/reaction for the internal control (shown in the CY5 plot).
PlexTaqreg; 5x qPCR Multiplex Master Mixnbsp;contains all components necessary for rapid, sensitive and reproducible quantification of DNA and cDNA. An engineered DNA polymerase and an optimized buffer including ultrapure dNTPs are key components of the ready to use mix. A hot-start formulation of the included DNA polymerase preventsnbsp;aptamer prevents false amplification and provides a fast start function.nbsp;
PlexTaqreg;acute;s formulation allows to use it also for direct PCRs from crude samples.