GreenDye 20x
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Boost your qPCR results without inhibition with GreenDye!
GreenDye is a highly sensitive DNA binding dye suitable for qPCR applications and melting curve analysis.
GreenDye shows very low DNA polymerase inhibition when compared with other dyes in PCR. It features a very high fluorescence when bound to double-stranded DNA and exhibits no degradation during PCR cycling. It is a perfect dye for qPCR and high resolution melting applications.For research use and further manufacturing.
In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.
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- real-time PCR (FAM channel)
- melt curve analysis
- High Resolution Melting (HRM)
- qPCR
- real-time primer extension
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For example, add 1.25 μl GreenDye to a 25 µl PCR reaction. The fluorescence is measured at the usual SYBR/FAM channels. -
This product is shipped in cool packs. Please store the product upon arrival at - 20 °C. Minimize the number of freeze-thaw cycles by storing in aliquots. For a day-to-day use, we recommend keeping an aliquot at 4 °C.
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GreenDye 20x is tested together with HiDi DNA polymerase (#9001) in a real-time PCR. A sequence in genomic HeLa DNA is used as a target to validate ct- and fluorescence values of GreenDye at 1x concentration.
Please inquire more information at info@mypols.de for lot-specific information.
No contamination has been detected in standard test reactions.
Benchmarking GreenDye against competitors
The fluorescence of GreenDye is benchmarked using a qPCR assay for the detection of a sequence in genomic HeLa DNA. PCRs were performed with 1 ng/µl of HeLa gDNA in the presence of either GreenDye 1x, EvaGreen® 1x or SYBR® Green I 1x in combination with HiDi DNA polymerase.
The real-time PCR curve with 1x GreenDye (green) in comparison with 1x EvaGreen (grey) and 1x SYBR® Green I (blue) shows the lowest ct-value.
GreenDye Melt Curve Analysis
The melting curves are acquired from the PCR experiment described above. All three dyes show a single peak indicating a single amplification product. GreenDye however, shows the sharpest melting peak.
