Isotherm3G is a mesophilic DNA polymerase, perfectly suited for Isothermal amplifications. Isotherm3G has been mutated to have improved reverse transcription activity. It synthesizes DNA from both DNA and RNA templates with a high strand displacement activity allowing simplified one-enzyme RT-LAMP reactions.
Isotherm3G exhibits 5’ to 3’ polymerase activity but lacks any exonuclease activity. The product components have been optimized and are perfectly suited for loop-mediated isothermal amplification (LAMP) with optimal reaction temperature at 65°C.
The product is available also in bulk quantities with custom fillings and can be produced customized for example in a lyo-ready formulation.
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Isotherm3G DNA polymerase is supplied as a 8 U/µl solution containing glycerol. It is supplied with a 10x reaction buffer which has been optimized for isothermal amplifications. The optimal magnesium, primer and dNTPs concentration strongly depends on the applied application and should be optimized.
This product is shipped on cool packs. It is recommended to store the product upon arrival at -20°C.
Our quality management system is certified according to ISO 13485.
myPOLS Biotec is part of Medix Biochemica Group.
Phone: +49 (0)7531 122 965 00
Fax: +49 (0)7531 122 965 02
You find us at Byk-Gulden-Straße 2
78467 Konstanz, Germany
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