Taq 2x PCR Master Mix - ready to use mix simplifies your PCR setup. Only primers and template need to be added as the mix contains all copmponents for a successful and reliable PCR. This ensures reproducible results, significantly reduces set-up times and the risk of pipetting errors. The robustness of PCR performance allows the application of this mix in a wide range of PCR amplifications.
For research use and further manufacturing.
In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.
This mix contains a Taq DNA polymerase variant in reaction buffer and ultrapure dNTPs. The DNA polymerase is hotstart-formulated with an aptamer. Temperatures above 50-55°C cause the aptamer’s secondary structure to melt and will set-free the DNA polymerase. It can also be used for real-time cycling, when adding a suitable realtime dye, for example GreenDye (#2000), or a fluorescent probe.
This product is shipped on cool packs. It is recommended to store the product upon arrival at -20°C.
Taq 2x PCR Master Mix is tested for successful PCR performance. A 92 bp fragment (beta-actin gene) was amplified from human genomic DNA and analysed by agarose gel electrophoresis. The activity of Taq DNA polymerase was monitored and adjusted to a specific DNA polymerase activity using an artificial DNA template and DNA primer. Enzyme concentration was determined by protein-specific staining. Please inquire more information at firstname.lastname@example.org for the lot-specific concentration. No contamination was detected in standard test reactions.
Different-sized amplicons from 3 ng of a DNA plasmid were amplified. The use of Taq DNA polymerase resulted in clean and high yield of products, as analysed after PCR on a 0.8% agarose gel.
A fragment (64 bp) of the human blood-coagulation factor IIa (F2) was amplified from 20 ng, 2 ng, 200 pg and 20 pg of a human genomic DNA extract. The same experiment was performed in parallel using the Taq DNA polymerase mix from another well-established and known supplier. PCR products were subsequently analysed on a 2.5% agarose gel.