Isotherm3G DNA Polymerase
-
Isotherm3G is a mesophilic DNA polymerase, perfectly suited for Isothermal amplifications. Isotherm3G has been mutated to have improved reverse transcription activity. It synthesizes DNA from both DNA and RNA templates with a high strand displacement activity allowing simplified one-enzyme RT-LAMP reactions.
Isotherm3G exhibits 5’ to 3’ polymerase activity but lacks any exonuclease activity. The product components have been optimized and are perfectly suited for loop-mediated isothermal amplification (LAMP) with optimal reaction temperature at 65°C.
Isotherm3G DNA Polymerase utilizes an aptamer-based warm-start feature to prevent false amplification at low temperatures. For more information, please contact us.
The product is available also in bulk quantities with custom fillings and can be produced customized for example in a lyo-ready formulation.
> download product flyer -
Isotherm3G DNA polymerase is supplied as a 8 U/µl solution containing glycerol. It is supplied with a 10x reaction buffer which has been optimized for isothermal amplifications. The optimal magnesium, primer and dNTPs concentration strongly depends on the applied application and should be optimized.
-
This product is shipped on cool packs. It is recommended to store the product upon arrival at -20°C.
