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Isotherm3G DNA Polymerase

  • Isotherm3G is a mesophilic DNA polymerase, perfectly suited for Isothermal amplifications. Isotherm3G has been mutated to have improved reverse transcription activity. It synthesizes DNA from both DNA and RNA templates with a high strand displacement activity allowing simplified one-enzyme RT-LAMP reactions.

    Isotherm3G exhibits 5’ to 3’ polymerase activity but lacks any exonuclease activity. The product components have been optimized and are perfectly suited for loop-mediated isothermal amplification (LAMP) with optimal reaction temperature at 65°C.

    The product is available also in bulk quantities with custom fillings and can be produced customized for example in a lyo-ready formulation. 

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  • Isotherm3G DNA polymerase is supplied as a 8 U/µl solution containing glycerol. It is supplied with a 10x reaction buffer which has been optimized for isothermal amplifications. The optimal magnesium, primer and dNTPs concentration strongly depends on the applied application and should be optimized.

  • This product is shipped on cool packs. It is recommended to store the product upon arrival at -20°C.

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