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m6A sensitive RT-KTQ DNA Polymerase Mutant

  • Interested in the analysis of DNA or RNA modifications? Then this DNA polymerase could help to analyze such modifications. The m6A sensitive DNA polymerase  exhibits increased misincorporation rates opposite m6A, while unmodified adenine is not affected. This prevents the loss of methylation information during reverse transcription and thus allows direct m6A sequencing. For further information refer to the original publication.   

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    The m6A sensitive DNA polymerase is supplied as a 5 µM solution containing glycerol and is supplied together with 10x reaction buffer. 

    The enzyme can also be used for real-time cycling, when adding a suitable dye.

    Link to the original publication

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