PlexTaq® 5x qPCR Multiplex Master Mix
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All in One. All the flexibility you need in a 5x Master Mix for multiplexing applications. NOW LYO READY!
+ Sensitive. More free volume. 5x concentration allows more volume for target specific primers and probes (multiplexing).
+ Robust. Uniform amplification. Up to 30 target multiplexing in real-time (customer feedback).
+ Fast TTR. No extraction needed. Reliable results with crude samples without extraction step, like blood.
+ Specific. No false amplification. Engineered Taq DNA polymerase more stable at room temperature. Aptamer-based hot-start prevents false amplification and provides a fast-start function.
+ Lyo ready. Contains all necessary excipients needed for freeze-drying. Can be used for lyophilization. Enables RT storage and shipping once dried. Can be freeze-dried by us. Learn MoreFor research use and further manufacturing. Designed and manufactured under ISO13485.
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- Probe based Real time qPCRs
- Real time multiplex detection qPCRs
- Direct real-time qPCRs (without sample extraction)
Examples:
molecular species identification by DNA barcoding
multiplexed amplifications for NGS-based barcoding -
How can I optimize the PCR conditions and prevent false amplification?
- The annealing/extension temperature can usually be optimized. Try a temperature gradient and determine the best temperature, which results in a high amplification signal.
- Shorten the extension and annealing time - too long and too many cycles may lead to over-amplification and side-products.
How do I have to store the mix?
- Keep at -20°C for long-time storage
- Minimize the number of freeze-thaw cycles by storing the product in aliquots. For a day-to-day use, we recommend keeping an aliquot at 4°C. -
The activity of PlexTaq® DNA polymerase is monitored and adjusted to a specific DNA polymerase activity using an artificial DNA template and a DNA primer. PlexTaq® 5x qPCR Multiplex Master Mix is tested in a real-time multiplex qPCR. Hereby, the product demonstrates linearity of amplification over a specified serial dilution of target DNA. Enzyme concentration is determined by protein-specific staining. Please inquire more information at info@mypols.de for the lot-specific concentration. No contamination has been detected in standard test reactions.
PlexTaq® 5x qPCR Multiplex Master Mix is produced to our highest internal quality guidelines and within the scope of the actively held ISO 13485 quality management system certification.
Exemplary tetraplex PCR assay
Detection of three specific pathogen targets and an internal control. Total input DNA per reaction was 10^6 (red), 10^5 (yellow), 10^4 (blue), 10^3 (green), 10^2 (purple), 10 (light blue) and 0 copies (grey) and 8000 copies/reaction for the internal control (shown in the CY5 plot).
5x Multiplex - robust PCR perforemance for a wide range of qPCR application
PlexTaq 5x qPCR Multiplex Master Mix contains all components necessary for rapid, sensitive and reproducible quantification of DNA and cDNA. An engineered DNA polymerase and an optimized buffer including ultrapure dNTPs are key components of the ready to use mix. A hot-start formulation of the included DNA polymerase prevents aptamer prevents false amplification and provides a fast start function.
PlexTaq®´s formulation allows to use it also for direct PCRs from crude samples.
