Taq 2x PCR Master Mix - ready to use mix simplifies your PCR setup. Only primers and template need to be added as the mix contains all copmponents for a successful and reliable PCR. This ensures reproducible results, significantly reduces set-up times and the risk of pipetting errors. The robustness of PCR performance allows the application of this mix in a wide range of PCR amplifications.
For research use and further manufacturing.
This mix contains a Taq DNA polymerase variant in reaction buffer and ultrapure dNTPs. The DNA polymerase is hotstart-formulated with an aptamer. Temperatures above 50-55°C cause the aptamer’s secondary structure to melt and will set-free the DNA polymerase. It can also be used for real-time cycling, when adding a suitable realtime dye, for example GreenDye (#2000), or a fluorescent probe.
This product is shipped on cool packs. It is recommended to store the product upon arrival at -20°C.
Taq 2x PCR Master Mix is tested for successful PCR performance. A 92 bp fragment (beta-actin gene) was amplified from human genomic DNA and analysed by agarose gel electrophoresis. The activity of Taq DNA polymerase was monitored and adjusted to a specific DNA polymerase activity using an artificial DNA template and DNA primer. Enzyme concentration was determined by protein-specific staining. Please inquire more information at firstname.lastname@example.org for the lot-specific concentration. No contamination was detected in standard test reactions.
Different-sized amplicons from 3 ng of a DNA plasmid were amplified. The use of Taq DNA polymerase resulted in clean and high yield of products, as analysed after PCR on a 0.8% agarose gel.
A fragment (64 bp) of the human blood-coagulation factor IIa (F2) was amplified from 20 ng, 2 ng, 200 pg and 20 pg of a human genomic DNA extract. The same experiment was performed in parallel using the Taq DNA polymerase mix from another well-established and known supplier. PCR products were subsequently analysed on a 2.5% agarose gel.