Non-specific amplification is prevented by using this Taq DNA polymerase variant as the DNA polymerase is hotstart-formulated. The 10x reaction buffer supplied together with the DNA polymerase has been specifically designed for optimal PCR performance and DNA polymerase activity. This allows the use of this DNA polymerase in a wide range of PCR applications.
For research use and further manufacturing. Designed and manufactured under ISO13485.
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The DNA polymerase is hotstart-formulated with an aptamer. Temperatures above 50-55°C cause the aptamer’s secondary structure to melt and will set-free the DNA polymerase. The DNA polymerase is supplied together with 10x reaction buffer. It can also be used for real-time cycling, when adding a suitable realtime dye, for example GreenDye (#2000), or a fluorescent probe.
This product is shipped on cool packs. It is recommended to store the product upon arrival at -20°C.
Taq DNA polymerase is tested for successful PCR performance. A 92 bp fragment (beta-actin gene) was amplified from human genomic DNA and analysed by agarose gel electrophoresis. The activity of Taq DNA polymerase was monitored and adjusted to a specific DNA polymerase activity using an artificial DNA template and DNA primer. Enzyme concentration was determined by protein-specific staining. Please inquire more information at firstname.lastname@example.org for the lot-specific concentration. No contamination was detected in standard test reactions.
Different-sized amplicons from 3 ng of a DNA plasmid were amplified. The use of Taq DNA polymerase resulted in clean and high yield of products, as analysed after PCR on a 0.8% agarose gel.
A fragment (64 bp) of the human blood-coagulation factor IIa (F2) was amplified from 20 ng, 2 ng, 200 pg and 20 pg of a human genomic DNA extract. The same experiment was performed in parallel using the Taq DNA polymerase mix from another well-established and known supplier. PCR products were subsequently analysed on a 2.5% agarose gel.